Article
A set of powerful negative selection systems for unmodified Enterobacteriaceae.
Nucleic acids research - 27 Jul 2015
Khetrapal Varnica, Mehershahi Kurosh, Rafee Shazmina, Chen Siyi, Lim Chiew Ling, Chen Swaine L
Abstract excerpt
Creation of defined genetic mutations is a powerful method for dissecting mechanisms of bacterial disease; however, many genetic tools are only developed for laboratory strains. We have designed a modular and general negative selection strategy based on inducible toxins that provides high selection stringency in clinical Escherichia coli and Salmonella isolates. No strain- or species-specific optimization is...
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