Article
Dominant negative inhibition by fragments of a monomeric enzyme.
Proceedings of the National Academy of Sciences of the United States of America - 10 Dec 1996
Michaels J E, Schimmel P, Shiba K, Miller W T
Abstract excerpt
Dominant negative inhibition is most commonly seen when a mutant subunit of a multisubunit protein is coexpressed with the wild-type protein so that assembly of a functional oligomer is impaired. By analogy, it should be possible to interfere with the functional assembly of a monomeric enzyme by interfering with the folding pathway. Experiments in vitro by others suggested that fragments of a monomeric enzyme...
Topics
- Amino Acyl-tRNA Synthetases
- Enzyme Repression
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Mutation
- Protein Folding