Article
A comparative analysis of perturbations caused by a gene knock-out, a dominant negative allele, and a set of peptide aptamers.
Molecular & cellular proteomics : MCP - 1 Dec 2007
Abed Nadia, Bickle Marc, Mari Bernard, Schapira Matthieu, Sanjuan-España Raquel, Robbe Sermesant Karine, Moncorgé Olivier, Mouradian-Garcia Sandrine, Barbry Pascal, Rudkin Brian B, Fauvarque Marie-Odile, Michaud-Soret Isabelle, Colas Pierre
Abstract excerpt
The study of protein function mostly relies on perturbing regulatory networks by acting upon protein expression levels or using transdominant negative agents. Here we used the Escherichia coli global transcription regulator Fur (ferric uptake regulator) as a case study to compare the perturbations exerted by a gene knock-out, the expression of a dominant negative allele of a gene, and the expression of peptide...
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