Article
Assessment of the utilization of the antisense RNA strategy to identify essential genes in heterologous bacteria.
FEMS microbiology letters - 28 Mar 2003
Wang Bing, Kuramitsu Howard K
Abstract excerpt
We employed an antisense RNA approach to identify essential genes common in both Gram-positive and Gram-negative bacteria by cloning a random library of Streptococcus mutans chromosomal DNA into an expression vector and transforming Escherichia coli. Twelve out of 27 E. coli transformants with growth defective phenotypes contained individual structural genes of S. mutans in the antisense orientation relative to...
Topics
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genes, Essential
- Gram-Negative Bacteria
- Gram-Positive Bacteria
- Models, Genetic
- Mutation
- RNA, Antisense
- Streptococcus mutans