Article
Disruption of the gene encoding the secreted acid protease (ACP) in the yeast Candida tropicalis.
FEMS microbiology letters - 15 Aug 1992
Sanglard D, Togni G, de Viragh P A, Monod M
Abstract excerpt
The gene for the secreted acid protease (ACP), a potential virulence factor of Candida species, was inactivated in Candida tropicalis by gene disruption. The disruption was performed by cotransformation of an ade2 C. tropicalis mutant with a linear DNA fragment carrying a deletion in ACP, and the replicative vector pMK16 which carries a selectable ADE2 gene marker. Few of the transformants exhibited lower...
Topics
- Aspartic Acid Endopeptidases
- Candida
- Chromosome Deletion
- Culture Media
- Gene Expression
- Mutation
- Serum Albumin, Bovine