Article
An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity.
Molecular microbiology - 1 Jun 1992
Tang C M, Cohen J, Holden D W
Abstract excerpt
Invasive pulmonary aspergillosis, usually caused by Aspergillus fumigatus, is a life-threatening condition of immunosuppressed patients. We have created a mutant strain of this fungus that lacks an extracellular alkaline protease (AFAlp). This was accomplished by transformation of A. fumigatus with a plasmid containing a selectable marker for hygromycin B resistance, and a 504 bp segment of the AFAlp gene,...
Topics
- Aspergillus fumigatus
- Base Sequence
- Molecular Sequence Data
- Mutation
- Pancreatic Elastase
- Transformation, Genetic