Article
High-resolution DNA melting curve analysis to establish HLA genotypic identity.
Tissue antigens - 1 Aug 2004
Zhou L, Vandersteen J, Wang L, Fuller T, Taylor M, Palais B, Wittwer C T
Abstract excerpt
High-resolution melting curve analysis is a closed-tube fluorescent technique that can be used for genotyping and heteroduplex detection after polymerase chain reaction. We applied this technique at the HLA-A locus and suggest that this method can be used as a rapid, inexpensive screen between siblings prior to living-related transplantation. At any locus, there are seven general cases of shared alleles among two...
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