Article
High-resolution melting analysis for genotyping Duffy, Kidd and Diego blood group antigens.
Legal medicine (Tokyo, Japan) - 1 Jan 2011
Tanaka Mitsunobu, Takahahi Junko, Hirayama Fumiya, Tani Yoshihiko
Abstract excerpt
High-resolution melting (HRM) analysis is a simpler genotyping method than allele-specific PCR, PCR-restriction fragment length polymorphism and multiplex PCR. Duffy, Kidd and Diego are clinically important blood group antigens. We used a novel method to genotype these three blood group antigens. Purified genomic DNA extracts of blood samples (354 Duffy, 347 Kidd and 457 Diego) were amplified using specific...
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