Article
Isolation of new temperature-sensitive mutants of Saccharomyces cerevisiae deficient in mannose outer chain elongation.
Yeast (Chichester, England) - 1 Jul 1992
Nagasu T, Shimma Y, Nakanishi Y, Kuromitsu J, Iwama K, Nakayama K, Suzuki K, Jigami Y
Abstract excerpt
We have isolated two temperature-sensitive Saccharomyces cerevisiae mutants which exhibit a deficiency in mannose outer chain elongation of asparagine-linked oligosaccharide. The size of yeast glycoprotein, secretory form of invertase, of one mutant (och1) was slightly larger than that of the sec...
Topics
- Asparagine
- Base Sequence
- Genetic Engineering
- Glycoside Hydrolases
- Mannose
- Molecular Sequence Data
- Mutation
- Nucleotide Mapping
- Oligosaccharides
- Phenotype
- Saccharomyces cerevisiae
- Temperature
- Tissue Plasminogen Activator
- beta-Fructofuranosidase