Article
OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongation of asparagine-linked oligosaccharides.
The EMBO journal - 1 Jul 1992
Nakayama K, Nagasu T, Shimma Y, Kuromitsu J, Jigami Y
Abstract excerpt
The Saccharomyces cerevisiae och1 mutant shows a deficiency in the mannose outer chain elongation at the non-permissive temperature. We have cloned the OCH1 gene by complementation of temperature sensitive (ts) phenotype for growth. The integrant of OCH1 gene in the yeast chromosome can complemen...
Topics
- Amino Acid Sequence
- Asparagine
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Carbohydrate Sequence
- Cloning, Molecular
- DNA, Fungal
- Fungal Proteins
- Glycosylation
- Mannose
- Mannosyltransferases
- Membrane Glycoproteins
- Membrane Proteins
- Molecular Sequence Data
- Phenotype
- Plasmids
- Precipitin Tests