Article
A novel Saccharomyces cerevisiae secretory mutant possesses a thermolabile phosphomannose isomerase.
Journal of bacteriology - 1 Mar 1991
Payton M A, Rheinnecker M, Klig L S, DeTiani M, Bowden E
Abstract excerpt
A temperature-sensitive mutant of Saccharomyces cerevisiae was identified which at the restrictive temperature of 37 degrees C is unable to secrete a number of cell wall-associated proteins and thus resembles previously reported sec mutants. In contrast to other sec mutants, however, both the temperature-sensitive growth and the secretion defects can be repaired by the addition of D-mannose to growth media. We...
Topics
- Hot Temperature
- Mannose
- Mannose-6-Phosphate Isomerase
- Mutation
- Protein Denaturation
- Saccharomyces cerevisiae