Article
A screen for yeast mutants with defects in the dolichol-mediated pathway for N-glycosylation.
Proceedings of the National Academy of Sciences of the United States of America - 15 Feb 1994
Roos J, Sternglanz R, Lennarz W J
Abstract excerpt
Dolichol in the form of dolichyl phosphate participates in the synthesis of N- and O-linked glycoproteins and phosphatidylinositol-linked proteins in the yeast Saccharomyces cerevisiae. In this organism, as well as in higher eukaryotes, a number of the enzymes in the polyisoprenoid and glycoprotein biosynthetic pathways have not been identified. In this study, we have developed a convenient, highly sensitive...
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