Article
In vivo derived HIV-1 nef gene products are heterogeneous and lack detectable nucleotide binding activity.
AIDS research and human retroviruses - 1 May 1992
Harris M, Hislop S, Patsilinacos P, Neil J C
Abstract excerpt
Multiple HIV-1 nef genes were cloned from lymphocyte DNA of asymptomatic seropositive individuals by polymerase chain reaction (PCR). Sequence analysis of these clones revealed a unique set of nef variants with premature terminations (PCRnef 1 and 6), mutations at sites of potential posttranslati...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Products, nef
- Genes, nef
- Genetic Variation
- Glutathione Transferase
- HIV Seropositivity
- HIV-1
- Humans
- Molecular Sequence Data
- Nucleotides
- Polymerase Chain Reaction
- Protein Binding
- Recombinant Fusion Proteins
- Sequence Alignment
- nef Gene Products, Human Immunodeficiency Virus