Article
CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jun 1993
Mariani R, Skowronski J
Abstract excerpt
PCR was used to clone isolates of the human immunodeficiency virus type 1 (HIV-1) nef gene directly from peripheral blood leukocytes of HIV-1-infected individuals. A transient expression system with human CEM T cells was used to assess the effect of nef on CD4 antigen expression on the cell surfa...
Topics
- Acquired Immunodeficiency Syndrome
- Alleles
- Amino Acid Sequence
- Base Sequence
- CD4 Antigens
- DNA, Viral
- Down-Regulation
- Gene Products, nef
- Genes, nef
- HIV-1
- Humans
- Leukocytes
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Open Reading Frames
- Polymerase Chain Reaction
- nef Gene Products, Human Immunodeficiency Virus