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Article

Design and isolation of temperature-sensitive mutants of Gal4 in yeast and Drosophila.

Journal of molecular biology - 27 Jul 2007

Mondal Kajari, Dastidar Antara Ghosh, Singh Guramrit, Madhusudhanan S, Gande Santosh Lakshmi, VijayRaghavan K, Varadarajan Raghavan

Abstract excerpt

Little is known about mechanisms responsible for the temperature-sensitive (ts) phenotype, or of the transferability of ts mutants of a specific gene between organisms. Using a structure-based approach, nine ts mutants of Gal4 were generated in yeast by mutating four DNA binding residues. Two of these nine yeast ts mutants were cloned into P element vectors under control of the Elav and GMR promoters and...

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Identifiers

PMID: 17553522
DOI: 10.1016/j.jmb.2007.05.035

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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