Site-directed mutagenesis of the Pseudomonas aeruginosa cytotoxin for probing toxic activity.

Abstract excerpt

The location of amino acids with direct influence on toxic activity in the pore-forming cytotoxin from Pseudomonas aeruginosa was tested by site-directed mutagenesis. Mutant fragments obtained by the polymerase-chain reaction were subcloned into a cytotoxin gene-bearing plasmid to minimize the possibility of amplification error. Our data suggest an important role of cysteines for toxic activity of cytotoxin....

Topics

• Bacteriophages
• Base Sequence
• Blotting, Western
• Cytotoxins
• Escherichia coli
• Genes, Viral
• Genetic Vectors
• Molecular Sequence Data
• Mutagenesis, Site-Directed
• Mutation
• Polymerase Chain Reaction