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Article

Site-directed mutagenesis of the Pseudomonas aeruginosa cytotoxin for probing toxic activity.

European journal of biochemistry - 1 Mar 1992

Xiong G, Lutz F

Abstract excerpt

The location of amino acids with direct influence on toxic activity in the pore-forming cytotoxin from Pseudomonas aeruginosa was tested by site-directed mutagenesis. Mutant fragments obtained by the polymerase-chain reaction were subcloned into a cytotoxin gene-bearing plasmid to minimize the possibility of amplification error. Our data suggest an important role of cysteines for toxic activity of cytotoxin....

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Source

Identifiers

PMID: 1541291
DOI: 10.1111/j.1432-1033.1992.tb16696.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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