Article
Method to protect a targeted amino acid residue during random mutagenesis.
Nucleic acids research - 15 Aug 2003
Umeno Daisuke, Hiraga Kaori, Arnold Frances H
Abstract excerpt
To generate a random mutant library that is free from mutation at a particular amino acid residue, we replace the codon of interest with a detachable, short DNA sequence containing a BsaXI recognition site. After PCR mutagenesis, this sequence is removed and intramolecular ligation of the sequences flanking the insert regenerates the gene. The three-base cohesive ends for ligation correspond to the codon for the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.