Article
Combinatorial library cloning of human antibodies to Streptococcus pneumoniae capsular polysaccharides: variable region primary structures and evidence for somatic mutation of Fab fragments specific for capsular serotypes 6B, 14, and 23F.
Infection and immunity - 1 Feb 2001
Lucas A H, Moulton K D, Tang V R, Reason D C
Abstract excerpt
Antibodies specific for capsular polysaccharides play a central role in immunity to encapsulated Streptococcus pneumoniae, but little is known about their genetics or the variable (V) region polymorphisms that affect their protective function. To begin to address these issues, we used combinatorial library cloning to isolate pneumococcal polysaccharide (PPS)-specific Fab fragments from two vaccinated adults. We...
Topics
- Adult
- Amino Acid Sequence
- Animals
- Antibodies, Bacterial
- Bacterial Capsules
- Base Sequence
- Cloning, Molecular
- Combinatorial Chemistry Techniques
- Female
- Humans
- Immunoglobulin Fab Fragments
- Immunoglobulin Variable Region