Article
Roles of Escherichia coli heat shock proteins DnaK, DnaJ and GrpE in mini-F plasmid replication.
Molecular & general genetics : MGG - 1 Jan 1990
Kawasaki Y, Wada C, Yura T
Abstract excerpt
A subset of Escherichia coli heat shock proteins, DnaK, DnaJ and GrpE were shown to be required for replication of mini-F plasmid. Strains of E. coli K12 carrying a missense mutation or deletion in the dnaK, dnaJ, or grpE gene were virtually unable to be transformed by mini-F DNA at the temperature (30 degrees C) that permits cell growth. When excess amounts of the replication initiator protein (repE gene...
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