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Article

Isolation and characterization of dnaJ null mutants of Escherichia coli.

Journal of bacteriology - 1 Sept 1990

Sell S M, Eisen C, Ang D, Zylicz M, Georgopoulos C

Abstract excerpt

Bacteriophage lambda requires the lambda O and P proteins for its DNA replication. The rest of the replication proteins are provided by the Escherichia coli host. Some of these host proteins, such as DnaK, DnaJ, and GrpE, are heat shock proteins. Certain mutations in the dnaK, dnaJ, or grpE gene block lambda growth at all temperatures and E. coli growth above 43 degrees C. We have isolated bacterial mutants that...

Topics

Bacterial Proteins
Bacteriophage lambda
DNA Replication
DNA, Bacterial
Electrophoresis, Gel, Two-Dimensional
Escherichia coli
Escherichia coli Proteins
Genotype
HSP40 Heat-Shock Proteins
Heat-Shock Proteins
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Plasmids

Restriction Mapping

Transduction, Genetic

Source

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Identifiers

PMID: 2144273
DOI: 10.1128/jb.172.9.4827-4835.1990
PMCID: PMC213136

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2144273-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Isolation and characterization of dnaJ null mutants of Escherichia coli.PMID 2144273Read ↗

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