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Article

The localized melting of mini-F origin by the combined action of the mini-F initiator protein (RepE) and HU and DnaA of Escherichia coli.

Molecular & general genetics : MGG - 27 Nov 1996

Kawasaki Y, Matsunaga F, Kano Y, Yura T, Wada C

Abstract excerpt

Replication of mini-F plasmids requires the initiator protein RepE, which binds specifically to four iterons within the origin (ori2), as well as some host factors that are involved in chromosomal DNA replication. To understand the role of host factors and RepE in the early steps of mini-F DNA replication, we examined the effects of RepE and the Escherichia coli proteins DnaA and HU on the localized melting of...

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Identifiers

PMID: 9003285
DOI: 10.1007/s004380050294

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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The localized melting of mini-F origin by the combined action of the mini-F initiator protein (RepE) and HU and DnaA of Escherichia coli.PMID 9003285Read ↗

Binding of RepE initiator protein to mini-F DNA origin (ori2). Enhancing effects of repE mutations and DnaJ heat shock protein.Semantic proximity Read ↗

DNA-binding domain of the RepE initiator protein of mini-F plasmid: involvement of the carboxyl-terminal region.Semantic proximity Read ↗

Regulation of DNA replication by iterons: an interaction between the ori2 and incC regions mediated by RepE-bound iterons inhibits DNA replication of mini-F plasmid in Escherichia coli.Semantic proximity Read ↗

The iteron regions necessary for the RepE-iteron interaction in vivo in mini-F plasmids of Escherichia coli.Semantic proximity Read ↗

Mini-F plasmid mutants able to replicate in Escherichia coli deficient in the DnaJ heat shock protein.Semantic proximity Read ↗

Crystal structure of a prokaryotic replication initiator protein bound to DNA at 2.6 A resolution.Semantic proximity Read ↗

Roles of Escherichia coli heat shock proteins DnaK, DnaJ and GrpE in mini-F plasmid replication.Semantic proximity Read ↗

Two new early bacteriophage T4 genes, repEA and repEB, that are important for DNA replication initiated from origin E.Semantic proximity Read ↗

Plasmid P1 RepA is homologous to the F plasmid RepE class of initiators.Semantic proximity Read ↗

Mini-F plasmid mutants able to replicate in the absence of sigma 32: mutations in the repE coding region producing hyperactive initiator protein.Semantic proximity Read ↗

Crystallization and preliminary X-ray diffraction studies of a replication initiator protein (RepE54) of the mini-F plasmid complexed with iteron DNA.Semantic proximity Read ↗

Sequential and ordered assembly of E1 initiator complexes on the papillomavirus origin of DNA replication generates progressive structural changes related to melting.Semantic proximity Read ↗

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