Binding of RepE initiator protein to mini-F DNA origin (ori2). Enhancing effects of repE mutations and DnaJ heat shock protein.

Article

Binding of RepE initiator protein to mini-F DNA origin (ori2). Enhancing effects of repE mutations and DnaJ heat shock protein.

The Journal of biological chemistry - 5 Jun 1992

Kawasaki Y, Wada C, Yura T

Abstract excerpt

Replication of mini-F plasmid in Escherichia coli requires the plasmid-encoded RepE initiator protein and a number of host factors and is regulated by interaction of RepE with specific sequences near the replication origin, ori2. We have examined DNA binding properties of several hyperactive muta...

Topics

Autoradiography
DNA, Bacterial
DNA-Binding Proteins
Escherichia coli Proteins
Heat-Shock Proteins
Mutation
Operator Regions, Genetic
Plasmids
Repressor Proteins
Restriction Mapping

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