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Article

Binding of RepE initiator protein to mini-F DNA origin (ori2). Enhancing effects of repE mutations and DnaJ heat shock protein.

The Journal of biological chemistry - 5 Jun 1992

Kawasaki Y, Wada C, Yura T

Abstract excerpt

Replication of mini-F plasmid in Escherichia coli requires the plasmid-encoded RepE initiator protein and a number of host factors and is regulated by interaction of RepE with specific sequences near the replication origin, ori2. We have examined DNA binding properties of several hyperactive mutant RepE proteins with single amino acid substitutions. Plasmids carrying these (repE) mutations, unlike the parental...

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Identifiers

PMID: 1597479

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Binding of RepE initiator protein to mini-F DNA origin (ori2). Enhancing effects of repE mutations and DnaJ heat shock protein.PMID 1597479Read ↗

Mini-F plasmid mutants able to replicate in Escherichia coli deficient in the DnaJ heat shock protein.Semantic proximity Read ↗

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Roles of Escherichia coli heat shock proteins DnaK, DnaJ and GrpE in mini-F plasmid replication.Semantic proximity Read ↗

The localized melting of mini-F origin by the combined action of the mini-F initiator protein (RepE) and HU and DnaA of Escherichia coli.Semantic proximity Read ↗

Mini-F plasmid mutants able to replicate in the absence of sigma 32: mutations in the repE coding region producing hyperactive initiator protein.Semantic proximity Read ↗

Regulation of DNA replication by iterons: an interaction between the ori2 and incC regions mediated by RepE-bound iterons inhibits DNA replication of mini-F plasmid in Escherichia coli.Semantic proximity Read ↗

Plasmid P1 RepA is homologous to the F plasmid RepE class of initiators.Semantic proximity Read ↗

Crystal structure of a prokaryotic replication initiator protein bound to DNA at 2.6 A resolution.Semantic proximity Read ↗

The iteron regions necessary for the RepE-iteron interaction in vivo in mini-F plasmids of Escherichia coli.Semantic proximity Read ↗

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Participation of the Escherichia coli heat shock proteins DnaJ, DnaK, and GrpE in autorepression of the P1 plasmid repA promoter.Semantic proximity Read ↗

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