Article
Role of calcineurin B homologous protein in pH regulation by the Na+/H+ exchanger 1: tightly bound Ca2+ ions as important structural elements.
Biochemistry - 30 Mar 2004
Pang Tianxiang, Hisamitsu Takashi, Mori Hidezo, Shigekawa Munekazu, Wakabayashi Shigeo
Abstract excerpt
We studied the role of the interaction of calcineurin homologous protein 1 (CHP1) with the Na(+)/H(+) exchanger 1 (NHE1), particularly its EF-hand Ca(2+) binding motifs, in the intracellular pH (pH(i))-dependent regulation of NHE1. We found that (45)Ca(2+) binds to two EF-hand motifs (EF3 and 4) of the recombinant CHP1 proteins with high affinity (apparent K(d) = approximately 90 nM). Complex formation between...
Topics
- Animals
- Binding Sites
- Calcineurin
- Calcium
- Calcium-Binding Proteins
- Cations, Divalent
- Cell Line
- Cricetinae
- EF Hand Motifs
- Humans
- Hydrogen-Ion Concentration
- Mutation
- Protein Binding
- Sequence Homology, Amino Acid