Article
Distinct roles for ATP binding and hydrolysis at individual subunits of an archaeal clamp loader.
The EMBO journal - 24 Mar 2004
Seybert Anja, Wigley Dale B
Abstract excerpt
Circular clamps are utilised by replicative polymerases to enhance processivity. The topological problem of loading a toroidal clamp onto DNA is overcome by ATP-dependent clamp loader complexes. Different organisms use related protein machines to load clamps, but the mechanisms by which they utilise ATP are surprisingly different. Using mutant clamp loaders that are deficient in either ATP binding or hydrolysis...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Catalysis
- DNA Polymerase III
- DNA, Archaeal
- DNA-Binding Proteins
- Euryarchaeota
- Hydrolysis
- Models, Biological
- Mutation
- Nucleotides
- Proliferating Cell Nuclear Antigen
- Protein Subunits
- Replication Protein C