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Article

Stepwise assembly of the human replicative polymerase holoenzyme.

eLife - 2 Apr 2013

Hedglin Mark, Perumal Senthil K, Hu Zhenxin, Benkovic Stephen

Abstract excerpt

In most organisms, clamp loaders catalyze both the loading of sliding clamps onto DNA and their removal. How these opposing activities are regulated during assembly of the DNA polymerase holoenzyme remains unknown. By utilizing FRET to monitor protein-DNA interactions, we examined assembly of the human holoenzyme. The results indicate that assembly proceeds in a stepwise manner. The clamp loader (RFC) loads a...

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Share to Adenosine Triphosphate
Share to DNA Polymerase III
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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Fluorescence Resonance Energy Transfer

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Share to Proliferating Cell Nuclear Antigen

Share to Protein Multimerization

Share to Protein Structure, Quaternary

Source

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Identifiers

PMID: 23577232
DOI: 10.7554/eLife.00278
PMCID: PMC3614016

Genes

PCNA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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