Article
Stepwise assembly of the human replicative polymerase holoenzyme.
eLife - 2 Apr 2013
Hedglin Mark, Perumal Senthil K, Hu Zhenxin, Benkovic Stephen
Abstract excerpt
In most organisms, clamp loaders catalyze both the loading of sliding clamps onto DNA and their removal. How these opposing activities are regulated during assembly of the DNA polymerase holoenzyme remains unknown. By utilizing FRET to monitor protein-DNA interactions, we examined assembly of the human holoenzyme. The results indicate that assembly proceeds in a stepwise manner. The clamp loader (RFC) loads a...
Topics
- Adenosine Triphosphate
- Binding Sites
- DNA
- DNA Polymerase III
- DNA Replication
- Fluorescence Resonance Energy Transfer
- Holoenzymes
- Humans
- Microscopy, Fluorescence
- Models, Molecular
- Mutation
- Proliferating Cell Nuclear Antigen
- Protein Binding
- Protein Multimerization
- Protein Structure, Quaternary