Article
Detection of mutation delta F508 in the cystic fibrosis gene using allele-specific PCR primers and time-resolved fluorometry.
PCR methods and applications - 1 Nov 1992
Iitiä A, Høgdall E, Dahlen P, Hurskainen P, Vuust J, Siitari H
Abstract excerpt
A method to detect the main cystic fibrosis (CF) mutation delta F508 from dried blood spots, whole blood, or saliva using the polymerase chain reaction (PCR) and time-resolved fluorometry (TRF) is described. Samples are treated by boiling in mild alkaline solution, after which two allele-specific...
Topics
- Alleles
- Base Sequence
- Blood Proteins
- Blood Specimen Collection
- Cystic Fibrosis
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA
- DNA Mutational Analysis
- Fluorometry
- Heterozygote
- Hot Temperature
- Humans
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligodeoxyribonucleotides
- Oligonucleotide Probes
- Polymerase Chain Reaction