Article
Detection of mutation delta F508 in the cystic fibrosis gene using allele-specific PCR primers and time-resolved fluorometry.
PCR methods and applications - 1 Nov 1992
Iitiä A, Høgdall E, Dahlen P, Hurskainen P, Vuust J, Siitari H
Abstract excerpt
A method to detect the main cystic fibrosis (CF) mutation delta F508 from dried blood spots, whole blood, or saliva using the polymerase chain reaction (PCR) and time-resolved fluorometry (TRF) is described. Samples are treated by boiling in mild alkaline solution, after which two allele-specific PCR reactions are performed. Allele-specific primers and a common biotinylated primer are used in the amplification...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.