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Article

Allele specific PCR with microfluorometry: application to the detection of del F508 mutation in cystic fibrosis.

Clinica chimica acta; international journal of clinical chemistry - 1 Feb 2002

Yamaguchi Akihiro, Nepote Juan Alberto, Kadivar Maliheh, Tagami Yasuko, Fukushi Masaru, Kikuchi Yuko, Sato Noriyuki, Kikuchia Kokichi

Abstract excerpt

BACKGROUND: A simple and practical screening method, allowing the mass detection of targeted DNA mutations, was developed by combined use of allele specific PCR (ASP) and subsequent fluorogenic intercalation to the amplicon. METHODS: Crude DNAs were extracted from dried blood spots (DBS) by a simple boil method. Highly specific polymerase chain reaction (PCR) amplification was achieved by adopting Taq DNA...

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PMID: 11750285
DOI: 10.1016/s0009-8981(01)00748-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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