Article
Suppression of spontaneous and hydrogen peroxide-induced mutations by a MutT-type nucleotide pool sanitization enzyme, the Escherichia coli Orf135 protein.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Dec 2003
Kamiya Hiroyuki, Iida Emiko, Murata-Kamiya Naoko, Yamamoto Yoshihiro, Miki Takeyoshi, Harashima Hideyoshi
Abstract excerpt
BACKGROUND: We recently found that the Escherichia coli Orf135 protein, a MutT-type enzyme, hydrolysed 2-hydroxy-dATP (2-OH-dATP), and less efficiently, 8-hydroxy-dGTP. RESULTS: In this study, we examined the effects of the absence of the orf135 gene. Frequencies of spontaneous and H2O2-induced mutations were two- to three-fold higher in the orf135- strain than in the wild-type strain. These mutations include...
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