Article
Increased A:T-->C:G mutations in the mutT strain upon 8-hydroxy-dGTP treatment: direct evidence for MutT involvement in the prevention of mutations by oxidized dGTP.
Journal of biochemistry - 1 Sept 2004
Kamiya Hiroyuki, Ishiguro Chieko, Harashima Hideyoshi
Abstract excerpt
The Escherichia coli MutT protein hydrolyzes 8-hydroxy-dGTP (8-OH-dGTP) in vitro, and mutT gene deficiencies cause increased spontaneous A:T-->C:G mutations. However, no direct evidence exists for enhanced mutagenicity of 8-OH-dGTP in mutT cells. In this study, 8-OH-dGTP was introduced into wild type and mutT E. coli cells, and mutations of a chromosomal gene were monitored. 8-OH-dGTP induced mutations of the...
Topics
- Adenosine Triphosphate
- DNA Mutational Analysis
- DNA-Directed RNA Polymerases
- Deoxyribonucleotides
- Escherichia coli
- Escherichia coli Proteins
- Guanosine Triphosphate
- Mutation
- Oxygen
- Phosphoric Monoester Hydrolases
- Pyrophosphatases