Article
Fluorescent detection and isolation of DNA variants using stabilized RecA-coated oligonucleotides.
Genome research - 1 Jan 2004
Rice Michael C, Heckman Brandy M, Liu Yi, Kmiec Eric B
Abstract excerpt
Several genome resequencing strategies have been developed to detect genetic variation in populations and correlate diversity with phenotypic consequences. Commonly used methods of detecting single nucleotide polymorphisms (SNPs) use PCR amplification and indirect analysis, which can create template biases and enable user contamination. Here we present a novel assay to detect and isolate DNA variants using...
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