Article
DNA diagnostics by surface-bound melt-curve reactions.
The Journal of molecular diagnostics : JMD - 1 Feb 2007
Strömqvist Meuzelaar Linda, Hopkins Katie, Liebana Ernesto, Brookes Anthony J
Abstract excerpt
Melting-curve procedures track DNA denaturation in real time and so provide an effective way of assessing sequence variants. Dynamic allele-specific hybridization (DASH) is one such method, based on fluorescence, which uses heat to denature a single allele-specific probe away from one strand of a polymerase chain reaction product attached to a solid support. DASH is a proven system for research genotyping, but...
Topics
- Base Sequence
- DNA
- DNA Gyrase
- DNA Mutational Analysis
- DNA Primers
- Evaluation Studies as Topic
- Humans
- Molecular Diagnostic Techniques
- Molecular Sequence Data
- Nucleic Acid Denaturation
- Nucleic Acid Hybridization
- Polymorphism, Single Nucleotide
- Salmonella
- Sequence Alignment
- Sequence Analysis, DNA
- Transition Temperature