Article
Marking of specific sequences in double-stranded DNA molecules--SNP detection and direct observation.
Genome research - 1 Dec 2004
Shigemori Yasushi, Haruta Hirotaka, Okada Takao, Oishi Michio
Abstract excerpt
In this study, we describe a simple method to mark specific sequences in double-stranded DNA molecules. For the marking, we used two specifically designed oligonucleotides, one of which is complementary to the sequence to be marked and the other, serving as a splint, to make the marking stable and detectable by subsequent various analytical means. In the presence of the two deoxyoligonucleotides, whereas RecA...
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