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Article

Single-nucleotide polymorphism discovery by targeted DNA photocleavage.

Proceedings of the National Academy of Sciences of the United States of America - 28 Sept 2004

Hart Jonathan R, Johnson Martin D, Barton Jacqueline K

Abstract excerpt

Single-nucleotide polymorphisms are the largest source of genetic variation in humans. We report a method for the discovery of single-nucleotide polymorphisms within genomic DNA. Pooled genomic samples are amplified, denatured, and annealed to generate mismatches at polymorphic DNA sites. Upon photoactivation, these DNA mismatches are then cleaved site-specifically by using a small molecular probe, a bulky...

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Identifiers

PMID: 15383659
DOI: 10.1073/pnas.0406169101
PMCID: PMC521117

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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