Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Hybridization-induced dequenching of fluorescein-labeled oligonucleotides: a novel strategy for PCR detection and genotyping.

The American journal of pathology - 1 Jul 2003

Vaughn Cecily P, Elenitoba-Johnson Kojo S J

Abstract excerpt

Fluorescence-based detection methods are being increasingly utilized in molecular analyses. Sequence-specific fluorescently-labeled probes are favored because they provide specific product identification. The most established fluorescence-based detection systems employ a resonance energy transfer mechanism effected through the interaction of two or more fluorophores or functional groups conjugated to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Factor V
Share to Fluorescein
Share to Globins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hybridization, Genetic

Share to Methylenetetrahydrofolate Reductase (NADPH2)

Share to Oligonucleotide Probes

Share to Oxidoreductases Acting on CH-NH Group Donors

Share to Staining and Labeling

Source

Open in PubMed Open full text

Identifiers

PMID: 12819008
DOI: 10.1016/S0002-9440(10)63627-9
PMCID: PMC1868185

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Hybridization-induced dequenching of fluorescein-labeled oligonucleotides: a novel strategy for PCR detection and genotyping.PMID 12819008Read ↗

Genotyping of single nucleotide polymorphisms using base-quenched probe: a method does not invariably depend on the deoxyguanosine nucleotide.Semantic proximity Read ↗

Intrinsic deoxyguanosine quenching of fluorescein-labeled hybridization probes: a simple method for real-time PCR detection and genotyping.Semantic proximity Read ↗

A new ultrasensitive way to circumvent PCR-based allele distinction: direct probing of unamplified genomic DNA by solution-phase hybridization using two-color fluorescence cross-correlation spectroscopy.Semantic proximity Read ↗

Single-Labeled Oligonucleotides Showing Fluorescence Changes Upon Hybridization with Target Nucleic Acids.Semantic proximity Read ↗

Real-time PCR genotyping using displacing probes.Semantic proximity Read ↗

Synthesis of a fluorescence resonance energy transfer-based probe containing a tricyclic nucleoside analog for single nucleotide polymorphism typing.Semantic proximity Read ↗

Detection of the factor V Leiden mutation by a modified photo-cross-linking oligonucleotide hybridization assay.Semantic proximity Read ↗

Single-tube genotyping without oligonucleotide probes.Semantic proximity Read ↗

A single nucleotide polymorphism melt curve assay employing an intercalating dye probe fluorescence resonance energy transfer for forensic analysis.Semantic proximity Read ↗

DNA analysis by fluorescence quenching detection.Semantic proximity Read ↗

Blocking probe as a potential tool for detection of single nucleotide DNA mutations: design and performance.Semantic proximity Read ↗

HyBeacon probes for rapid DNA sequence detection and allele discrimination.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.