Article
Functional characterization of Candida albicans ABC transporter Cdr1p.
Eukaryotic cell - 1 Dec 2003
Shukla Suneet, Saini Preeti, Smriti, Jha Sudhakar, Ambudkar Suresh V, Prasad Rajendra
Abstract excerpt
In view of the importance of Candida drug resistance protein (Cdr1p) in azole resistance, we have characterized it by overexpressing it as a green fluorescent protein (GFP)-tagged fusion protein (Cdr1p-GFP). The overexpressed Cdr1p-GFP in Saccharomyces cerevisiae is shown to be specifically labeled with the photoaffinity analogs iodoarylazidoprazosin (IAAP) and azidopine, which have been used to characterize the...
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