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Article

Enhanced isolation of low frequency herpes simplex virus recombinants using green-fluorescent protein and FACS.

Journal of virological methods - 1 Jan 2004

Kolb Aaron W, Brandt Curtis R

Abstract excerpt

The generation of recombinant herpes simplex virus to study the effect of engineered mutations on viral biology relies on the isolation of recombinants from a mixed population of viruses following a marker transfer procedure. Currently, the E. coli lacZ or green-fluorescent protein (GFP) genes are most frequently used as markers for isolation and isolation of recombinants relies on visual screening of plaques....

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PMID: 14656463
DOI: 10.1016/j.jviromet.2003.09.017

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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