Article
Identification of recombinant baculoviruses using green fluorescent protein as a selectable marker.
BioTechniques - 1 Apr 1997
Wilson L E, Wilkinson N, Marlow S A, Possee R D, King L A
Abstract excerpt
A rapid procedure for the production and identification of recombinant baculoviruses is described that uses the autofluorescent properties of the Aquorea victoria green fluorescent protein (GFP). Expression of the GFP cDNA (without signal peptide sequence) in Spodoptera frugiperda cells resulted...
Topics
- Animals
- Baculoviridae
- Blotting, Western
- Cell Line
- DNA, Recombinant
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Fluorescence
- Gene Expression
- Genetic Markers
- Genetic Vectors
- Green Fluorescent Proteins
- Luminescent Proteins
- Microscopy, Fluorescence
- Phenotype
- Recombinant Proteins
- Spodoptera
- Ultraviolet Rays