Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells.

doi:10.1038/nbt0596-606

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Article

Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells.

Nature biotechnology - 1 May 1996

Cheng L, Fu J, Tsukamoto A, Hawley R G

Abstract excerpt

Two mutants of the green fluorescent protein (GFP), RSGFP4 and GFPS65T, have been recently created which differ from the wildtype GFP of A. victoria in their excitation maxima. Here we show that human fibroblasts transfected with either of the two mutant GFP genes emit a green fluorescence that i...

Topics

Animals
Biotechnology
Cell Line
Fluorescence
Gene Expression
Gene Transfer Techniques
Genetic Variation
Genetic Vectors
Green Fluorescent Proteins
Humans
Luminescent Proteins
Mice
Mutation
Retroviridae
Transduction, Genetic
Transfection

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