Article
A genetic selection method for the transfer of HSV-1 glycoprotein B mutations from plasmid to the viral genome: preliminary characterization of transdominance and entry kinetics of mutant viruses.
Virology - 1 Oct 1994
Desai P, Homa F L, Person S, Glorioso J C
Abstract excerpt
A genetic selection procedure has been devised to transfer mutations from a plasmid to the viral genome. Reagents were constructed so that the recombination events that occur during cotransfection rescue a mutation in a recipient viral genome and, simultaneously, transfer a mutation in an adjacen...
Topics
- Cell Line
- DNA, Viral
- Gene Expression
- Genes, Dominant
- Genes, Viral
- Genetic Engineering
- Genetic Markers
- Genome, Viral
- Herpesvirus 1, Human
- Kinetics
- Mutation
- Plasmids
- Recombination, Genetic
- Viral Envelope Proteins
- Viral Proteins
- Viral Structural Proteins
- Virion