Article
Applications of green fluorescent protein as a marker of retroviral vectors.
Somatic cell and molecular genetics - 1 Sept 1997
Kandel E S, Chang B D, Schott B, Shtil A A, Gudkov A V, Roninson I B
Abstract excerpt
The Green Fluorescent Protein (GFP) of Aequorea victoria is used as a vital fluorescent tag for the detection and isolation of genetically modified cells. Several modified variants of GFP were tested as marker genes in retroviral vectors containing different backbones and promoter combinations. Constructs allowing for reliable detection of GFP fluorescence and the expression of a cotransduced gene from a strong...
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