Article
Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Wu Hsuan-Chen, Cha Hyung Joon, Bentley William E
Abstract excerpt
By use of a strategy incorporating the green fluorescent protein (GFP), facile and rapid monitoring and visualization of baculovirus infection in insect cells is possible in vivo. This chapter describes two techniques for simple determination of virus titer in the baculovirus expression system using GFP co-expression and rapid monitoring of Sf-9 insect cell infection using a combination of GFP and the...
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