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Article

An efficient method for generating poxvirus recombinants in the absence of selection.

Viruses - 1 Mar 2011

Rice Amanda D, Gray Stacey A, Li Yu, Damon Inger, Moyer Richard W

Abstract excerpt

The use of selectable markers (ecogpt) and selection pressures to aid in detection of poxvirus (Vaccinia, VV) recombinants has been implicated in the unintended introduction of second site mutations. We have reinvestigated the use of the helper virus system described by Scheiflinger et al. and adapted by Yao and Evans which produces recombinants at a high frequency in the absence of any selection, at a rate of...

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Identifiers

PMID: 21494427
DOI: 10.3390/v3030217
PMCID: PMC3075091

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Source receipt: pubmed, retrieved 7 Sept 2026.

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