Article
Novel methyltransferase for modified uridine residues at the wobble position of tRNA.
Molecular and cellular biology - 1 Dec 2003
Kalhor Hamid R, Clarke Steven
Abstract excerpt
We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results in the complete...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA, Fungal
- Gene Deletion
- Genes, Fungal
- Methylation
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- RNA, Fungal
- RNA, Transfer
- RNA, Transfer, Arg
- RNA, Transfer, Glu
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Homology, Amino Acid
- Substrate Specificity
- Temperature