Article
Allele-specific methylation in the 5'-regulatory region of class II DQ beta genes in the human major histocompatibility complex (MHC): relationship to autoimmune disease susceptibility.
Disease markers - 1 Jan 2000
Toyoda H, Redford A, Magalong D
Abstract excerpt
The DNA methylation state of the 5'-regulatory region of human HLA-DQ beta genes was examined. Two restriction enzymes were utilized to detect methylated (meCG) dinucleotides in the 5'-regulatory region of the DQ-beta genes: the restriction enzyme Msp I, which recognizes CCGG and CmeCGG, and Hpa II recognizes only the unmethylated CG sequence. DNA samples were prepared from 95 HLA-typed individuals including 40...
Topics
- Alleles
- Autoimmune Diseases
- Diabetes Mellitus, Type 1
- Genes, MHC Class II
- Genes, Regulator
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- HLA-DR Antigens
- Humans
- Methylation