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Article

Isolation, characterization, and utilization of a temperature-sensitive allele of a Pseudomonas replicon.

Journal of microbiological methods - 1 Sept 2009

Silo-Suh Laura A, Elmore Brett, Ohman Dennis E, Suh Sang-Jin

Abstract excerpt

In order to facilitate genetic study of the opportunistic bacterial pathogen Pseudomonas aeruginosa, we isolated a conditional, temperature-sensitive plasmid origin of replication. We mutagenized the popular Pseudomonas stabilizing fragment from pRO1610 in vitro using the Taq thermostable DNA polymerase in a polymerase chain reaction (PCR). Out of approximately 23,000 potential clones, 48 temperature-sensitive...

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Identifiers

PMID: 19615413
DOI: 10.1016/j.mimet.2009.07.002
PMCID: PMC2782952

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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