Article
Detection of allele-specific expression of N-RAS oncogenes in human leukaemia cells.
British journal of haematology - 1 Jul 1992
Lübbert M, Mertelsmann R, Herrmann F
Abstract excerpt
We report analysis of allele-specific expression of N-RAS transcripts in myeloid leukaemic cells and cell lines. Expression was assessed by an assay of reverse-transcription/PCR combined with differential hybridization with mutation-specific oligonucleotides. In cells from all patients with acute myeloid leukaemia (AML) and in myeloid cell lines HL-60 and THP-1, expression of both the wild-type allele and the...
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