Article
Fidelity of HIV-1 reverse transcriptase copying RNA in vitro.
Biochemistry - 4 Feb 1992
Ji J P, Loeb L A
Abstract excerpt
The genomic hypervariation of human immunodeficiency virus 1 (HIV-1) could result from misincorporations by the viral reverse transcriptase. We developed an assay for reverse transcriptase fidelity during RNA-dependent as well as DNA-dependent DNA polymerization in vitro. A lacZ alpha RNA fragment transcribed by T3 RNA polymerase was used to mimic first-strand reverse transcription. The corresponding DNA template...
Topics
- Animals
- Base Sequence
- Cattle
- DNA, Viral
- HIV-1
- Molecular Sequence Data
- Mutation
- RNA, Viral
- RNA-Directed DNA Polymerase
- Templates, Genetic
- Transcription, Genetic