Role of murine leukemia virus reverse transcriptase deoxyribonucleoside triphosphate-binding site in retroviral replication and in vivo fidelity.

Abstract excerpt

Retroviral populations exhibit a high evolutionary potential, giving rise to extensive genetic variation. Error-prone DNA synthesis catalyzed by reverse transcriptase (RT) generates variation in retroviral populations. Structural features within RTs are likely to contribute to the high rate of errors that occur during reverse transcription. We sought to determine whether amino acids within murine leukemia virus...

Topics

• Amino Acid Sequence
• Binding Sites
• Cell Line
• Deoxyribonucleotides
• Genetic Vectors
• Lac Operon
• Leukemia Virus, Murine
• Molecular Sequence Data
• Mutation
• Plasmids
• RNA-Directed DNA Polymerase
• Retroviridae
• Transfection
• Virus Replication