Article
The base substitution fidelity of HIV-1 reverse transcriptase on DNA and RNA templates probed with 8-oxo-deoxyguanosine triphosphate.
Mutation research - 19 Oct 1999
Bebenek K, Boyer J C, Kunkel T A
Abstract excerpt
We have used 8-O-dGTP, a mutagenic nucleotide generated by oxidative metabolism, to probe the misincorporation potential of HIV-1 reverse transcriptase (RT) during DNA synthesis templated by the same nucleotide sequence as either RNA or DNA. With either template, 8-O-dGMP was misincorporated opposite template A, yielding characteristic A-->C transversions. The error rate with DNA was similar to that with RNA,...
Topics
- Base Pair Mismatch
- Base Sequence
- Binding Sites
- DNA
- Deoxyguanine Nucleotides
- HIV Reverse Transcriptase
- HIV-1
- Humans
- Models, Molecular
- Molecular Sequence Data
- Mutation