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Article

Gene construction and mutagenesis for site specific modification of protein with carbohydrate.

SAAS bulletin, biochemistry and biotechnology - 1 Jan 1990

Leung W C, Leung M F

Abstract excerpt

We reported the construction of the structural gene for trans-activator (Tat) protein of human immunodeficiency virus. While maintaining the same amino acid sequence as the viral protein, the corresponding nucleotide sequence was modified to create additional recognition sites for restriction endonucleases and to prevent basepair mismatch during gene assembly. The oligonucleotides were synthesized chemically,...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Viral
Gene Products, tat
Genes, Synthetic
Genes, Viral
HIV-1
Molecular Sequence Data
Mutation
Oligonucleotides

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Trans-Activators

Viral Structural Proteins

tat Gene Products, Human Immunodeficiency Virus

Source

Identifiers

PMID: 1366506

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1366506-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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