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Article

Gene construction and mutagenesis for site specific modification of protein with carbohydrate.

SAAS bulletin, biochemistry and biotechnology - 1 Jan 1990

Leung W C, Leung M F

Abstract excerpt

We reported the construction of the structural gene for trans-activator (Tat) protein of human immunodeficiency virus. While maintaining the same amino acid sequence as the viral protein, the corresponding nucleotide sequence was modified to create additional recognition sites for restriction endonucleases and to prevent basepair mismatch during gene assembly. The oligonucleotides were synthesized chemically,...

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PMID: 1366506

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